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Subject: protein-A antibody eluate pharm. The clarified culture medium was loaded onto a MabSelect SuRe (GE Healthcare) protein-A column and washed with 10 column volumes of PBS buffer at pH 7.2. The antibody was eluted with 10 column volumes of citrate buffer at pH 3.6 with the pooled fractions containing the antibody neutralized with TRIS at pH 11."The protein-A antibody eluate" was further purified by gel filtration (SEC). как перевести "The protein-A antibody eluate" - элюат антител, полученный с помощью белка А"? элюат, содержащий белок А и антитела? Спасибо! |
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