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Subject: slide/gaskets assemblies (в биологии) biol. HybridizationTwenty μL eluted sample was added robotically to an empty the 96-well plate. Five μL 10x Agilent blocking buffer containing a second set of hybridization controls were robotically added to the eluted samples. Then 25 μL 2x Agilent HiRPM hybridization buffer was added manually to the wells. 40 μL of hybridization mix was loaded onto the ""Agilent gasket slide"". The Agilent 8 by 15k array was added onto ""gasket slide"" and the sandwich was tightened with a clamp. The sandwich was then incubated rotating (20 rpm) for 19 hours at 55°C. Up to twelve "slide/gasket assemblies" were sequentially disassembled into the first staining dish containing Wash Buffer 1 and the slides were placed into a slide rack while still submerged in Wash Buffer 1. Once all "slide/gaskets assemblies "were disassembled, the slide rack was quickly transferred into Bath #1 of the Little Dipper Processor and the automated wash protocol was started. Спасибо! |
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